Scrimer is an automated pipeline for designing primers for genotyping from the next-generation sequence raw transcriptome data. The designed primers can be used in conjunction with several widely used genotyping methods such as SNaPshot or MALDI-TOF genotyping.
- Adapter removal
- Mapping reads
- SNP's selection
- Primer designing
Libor Mořkovský, Jan Pačes, Jakub Rídl and Radka Reifová (2015) Scrimer: designing primers from transcriptome data, Molecular Ecology Resources, 15(6): 1415-1420.